1629 High-throughput T cell receptor sequencing aids in the diagnosis of CTCL
نویسندگان
چکیده
Cutaneous T-cell lymphomas (CTCL) are difficult to diagnose and may resemble benign inflammatory dermatoses (BID). Hence, diagnosis requires consideration of clinical, histopathologic, molecular studies. Though, widely used studies rely on the T cell receptor g (TCRg) polymerase chain reaction (PCR) detect a clone, it is reportedly positive in ∼50% early CTCL cases. High throughput TCR sequencing (HTS) TCRb gene (ImmunoSEQ®) prior cohort has demonstrated more sensitive specific than TCRg PCR discriminates from BID. We evaluated performance clonoSEQ®, clinically available assay for detection dominate clone by HTS patients with suspected referred multi-disciplinary cutaneous lymphoma clinic at DFCI 2016-2021. A total 151 underwent clonoSEQ® skin biopsies, which 82 were considered clinicians meet criteria based all data. Among who also (n= 48), 97% had compared 79% PCR. single patient was deemed clinician have CTCL, despite absence later re-diagnosed tinea corporis complete remission their eruption following course topical anti-fungals. 44 1 38 2 clones, 6 3 4 clone. Given that an individual biallelic rearrangement TCRB (i.e. two clones derive one cell) we observed while largely monoclonal neoplastic process, select individuals oligoclonal cells. It not clear whether additional or reactive (e.g. anti-tumor cells).
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2023
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2023.03.1647